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KMID : 0370220100540060449
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Yakhak Hoeji
2010 Volume.54 No. 6 p.449 ~ p.454
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Structural Analysis of Oligosaccharides of a Plant Glycoprotein
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Bae Jae-Woo
Park Byung-Tae
Yoon Doo-Chun
Kim Joo-Young
Hwang Hye-Sung
Park Hyun-Joo
Na Jong-Chun
Kim Ha-Hyung
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Abstract
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The glycosylation of glycoproteins from mammalian or plants can affect their efficacy, stability, solubility, and half-life. In the present study, we investigated plant glycosylation and their relative intensity (%) in a plant carbohydratebinding protein with the hemagglutination and antiproliferative activities. The hemagglutination activity on the deglycosylated protein was decreased as a 16-fold than that of intact glycoprotein. Using the HPLC with fluorescence detector and mass spectrometer, the major eight bi- or triantennary oligosaccharides containing xylose, fucose, mannose, galactose, and N-acetylglucosamine were identified and structurally characterized. The present results indicate that the oligosaccharides on this plant glycoprotein is necessary for their own property.
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KEYWORD
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plant glycoprotein, oligosaccharide, structure
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